We now have validated fluorescence-based assays for >167 protein kinases. View the validated assays.
Contact us today to see if your target kinase is on our list!
We are a Life Science company founded by scientists that develops innovative enzyme assay formats for a quantum improvement in performance and productivity. We apply rigorous development, validation and manufacturing practices to create products that deliver exceptional value (easier, faster, better, cheaper) with expert customer support.
Real-Time Sensors of Protein Kinase Activity
Our initial focus is on assays for measuring the activity of the >500 protein kinases, which are dysregulated in many disease states and comprise >30% of all drug development. To do this, we have harnessed chelation-enhanced fluorescence (CHEF) using the sulfonamido-oxine (Sox) chromophore in peptide or protein substrates, to create real-time sensors of phosphorylation, as developed by the Imperiali laboratory at MIT. The result is a simple yet powerful method to measure the activity of protein kinases using a homogeneous and continuous (kinetic) format, where the level of fluorescence is directly proportional to the amount of phosphorylated product. This assay is ideal for elucidating drug mechanism of action and is increasingly being applied earlier in the drug development workflow to address the challenges and opportunities for next generation protein kinase inhibitors.
The Sox technology is covered by several patents and has been exclusively licensed by MIT to AssayQuant Technologies, Inc.
Workflow for Measuring Protein Kinase Activity Using Sox Technology
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This homogeneous and continuous (kinetic) format can be used with physiological (mM) ATP and with both purified enzymes or crude lysates.
Next Generation Sox Technology Sensors
At AssayQuant Technologies, Inc., we are actively developing next generation Sox Technology Sensors to meet the needs of our customers. These efforts incorporate improvements from the Imperiali laboratory, including the Cysteine-Sox method to create superior Sox-based substrates by allowing for flanking sequence recognition determinants on either side of the Sox moiety/phosphorylation site. We are applying this approach to generate both highly generic substrates (for use with a range of purified kinases) or highly-selective substrates (for use with unfractionated cell or tissue lysates; see Peterson et. al., 2014).
For a current list of Sox-based protein kinase assays or to discuss custom assay development for your targets of interest, please send us a message using the link provided below. We look forward to hearing from you as we continue to develop our Web site.
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