Harness the Power of Your Data With a Continuous Kinetic Assay Format

When a progress curve is linear from the beginning, and remains linear throughout the experiment, a single end point reading can approximate the reaction rate. However, frequently when interrogating protein kinases and their inhibitors, there is a delay in the onset of the reaction, or other changes in reaction rate over time. In these cases, important information about the reaction can be missed or mischaracterized in an endpoint assay. Using a continuous assay format, like our PhosphoSens^® technology, will visualize and quantify enzyme activity with confidence.

While visual inspection is the most common method of determining the range of the progress curve from which to extract rate information, this method is not practical when thousands of progress curves are generated in an experimental day. An automated method is essential for high throughput assessment of enzyme activity, for example in large-scale lead optimization or selectivity assessment, both of which are essential for developing cutting edge drug candidates.

Our fully automated linear range finding algorithm substantially increases the speed, reliability, and reproducibility of linear range definition for enzyme kinetic studies. The method enables high throughput screening in a kinetic enzyme assay, for example making possible rapid analysis of very large data sets from kinome profiling data.