Continuous, Kinetic Fluorescent Intensity (FI) Assays
PhosphoSens-Kinetic Assays: Capture real-time, kinetic data throughout the entire enzyme reaction at the substrate level, where the level of fluorescence is directly proportional to the amount of phosphorylated product.
Advantage: Continuous monitoring enables better characterization of reaction dynamics - ideal for studying enzyme inhibitors, activators, or drug effects - with higher accuracy and precision.
Endpoint Time-Resolved Fluorescence (TRF) Assays
PhosphoSens-Red Assays: Designed for highthoughput screening applications, capture a single fluorescence measurement without signal contamination, utilizing the same reaction conditions and set up as our Kinetic assays.
Advantage: TRF reads allow for clearer, more distinguishable signals, empowering researchers to conduct efficient, reliable, high-throughput kinase assays while retaining data quality and physiological relevance.
