PRODUCT

Kinetic Fluorescent Intensity (FI) Assays

Quantify kinase and phosphatase activity and inhibition in real-time in your lab with a simple add-and-read protocol

Continuous kinase & phosphatase assays with real-time, kinetic insights

Based on proprietary technology developed at MIT, PhosphoSens-Kinetic Assays deliver a new standard for measuring enzyme activity, providing continuous, direct monitoring throughout the entire reaction. These assays capture real-time data at the substrate level, offering richer insights into enzyme kinetics, inhibitor interactions, and reaction dynamics—without the limitations of traditional endpoint assays.

Continuous, Real-Time Monitoring

Captures the entire kinetic profile from start to finish. This approach yields the actual reaction rate, with high accuracy, precision, and confidence.

Direct Measurement of Enzyme Activity 

Measures enzyme activity at the substrate level, avoiding the complications of indirect assays that require additional steps.

Physiologically Relevant Conditions 

Use biologically relevant peptide substrate sequences in assays that are compatible with low to physiological [mM] concentrations of ATP.

Single-Step, Homogenous Workflow

Achieve fast and reproducible results with a homogenous, single-step workflow without compromising data quality.

PhosphoSens Catalog Product Packaging
PRODUCT CONFIGURATIONS

What's in the box?

PhosphoSens Substrates

Designed for higher throughput applications, each PhosphoSens Senor Peptide Substrate can be purchased as lyophilized 1mg aliquots. Each mg is sufficient for ~2,500 assays in a low‐volume 384‐well plate (10μM sensor peptide in 25μL reaction volume).

PhosphoSens Substrate Systems

Designed for higher throughput applications AND ease of use, each PhosphoSens Senor Peptide Substrate System pairs 1mg aliquots of substrate with the required reaction reagents needed to perform the assay (ATP, DTT, EGTA, Enzyme Reaction Buffer, and Enzyme Dilution Buffer). Each system is sufficient for ~2,500 assays in a low‐volume 384‐well plate (10μM sensor peptide in 25μL reaction volume).

PhosphoSens-Kinetic Discovery Kits

Designed for streamlined technology assessment, each discovery kit includes sufficient volumes of substrate and reaction reagents to perform 200 reactions in a low‐volume 384‐well plate (10μM sensor peptide in 25μL reaction volume).
*Discounted technology evaluation kits are available for first time users and for new projects. Please inquire.

 

 

 

 

 

 

NOTE: If there is a number designation after 'K-', the evaluation kit will contain the necessary additives. 

How it works

PhosphoSens continuous assays utilize a simple add-and-read protocol, providing the capability for real-time, kinetic measurements, with a straight-forward, fluorescence intensity (FI) measurement.

These assays provide high accuracy and precision by allowing measurement of the initial reaction rate (measured as the slope of the initial linear region), typically during the first 30-60 minutes and include many data points, with readings performed every 30 seconds to two minutes.

This continuous assay format also allows for correction of compound autofluorescence, because this background signal does not change over time and can be corrected for by subtracting background signals (includes compound and all components of the reaction besides the enzyme) from the total fluorescence (all components including enzyme) to determine the net signal at each time point.

The continuous format is compatible with essentially any commercially available fluorescence microplate reader capable of kinetic readings, and associated laboratory automation equipment. No stop solution or quench/develop step is needed, minimizing time, while maximizing throughput.

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How to Order

CREDIT CARD

Assays via credit card

Order PhosphoSens Substrates, Substrate Systems, and Discovery Kits online via credit card

VISIT ONLINE STORE
PURCHASE ORDER

Assays via purchase order

Inquire about purchasing any of our PhosphoSens Products or KinSight Services

REQUEST A QUOTE

Didn’t find what you were looking for?

We also offer custom assay development and kinase and phosphatase profiling services. Tell us about your specific project, and our experts can help you select the right PhosphoSens tool for your kinetic assay needs.

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